Gesellschaft zur Förderung von Medizin-,
Bio- und Umwelttechnologien e. V.

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This research area is concentrates on development and application of different biological based processes. It aims for the detection of single substances and summary parameters in liquide and gaseous samples (principle of Biosensors) or the physiological characterization of microorganisms, single cells and united cell structures (Bio-Activity-Sensors).

The basic principle of this applications is the Continuous-Flow Analysis. Additional to the detection methods the focus of development is on sampling systems and immobilization methods for biocompounds (cells, enzymes, antibodies).

Microbial Sensors

Biosensors are analytical devices that combine biological sensing elements with a transducer to generate a signal which is proportional to the analyte concentration. The transducer transforms the biological signal into a measurable response, which can be further amplified, processed and saved for analysis.

The tightly contact between living microorganisms and a transducer device is a precondition for its correct operation. Thus, the fabrication requires close immobilization of receptor on the transducer unit.

In order to develop selective microbial biosensors with a specific substrate spectrum it is essential to gain high specialized microorganisms.

The respiratory activity of the microorganisms is estimated by monitoring oxygen consumption using an automatic flow system. The microorganisms are immobilized on a membrane. Now it is possible to perform a phenol detection in liquid samples or a hydrogen sulfide detection in gases (e.g. biogas) in combination with suitable absorption techniques.

This technology can also be used to examine the influence of several light conditions on phototrophic microorganisms (e.g. microalgae) or plant tissue stuctures (leaf segments).

Enzyme sensors

Enzymes are immobilised covalent on porous spherical glass tracers and are used in flow-through reactors.

Currently a sensitive method based on enzyme catalyzed reactions is developed to detect diacetyl in beer matrices. Therefore intermediate catabolic products of specific enzymatic reactions are measured with fluorescence detection.

Furthermore reaction enthalpies of enzyme reactions can be detected in a microcalorimeter.

Bio-Activity-Sensors

The electrochemical bioactivity sensor (BAS) provides an alternative measuring system for determining the activity of microorganisms which is in contact with potential substrates. This principle can be used to characterize certain metabolic characteristics of a microorganism strain or a mixture of heterogenous microorganisms (e.g. aerobic sludges).

For this purpose potential subtrates are injected in a defined sequence. On the basis of the resulting signal pattern informations about the metabolic potential of the target strain can be devided (screening of substrates or adaption on specific conditions). Likewise, the effect of inhibitors or activators can simply be examined by this screening sensor.

To estimate contaminant content of water samples inhibition of microalgae photosynthetic oxygen release can be measured. In addition, photosynthetic active plant materials can be used to obtain informations about damages or limitations. Air sampling systems

The “Airsampler AS 10.1” is a universally usable, portable instrument for air sampling. This device allows the analytical assessment of air quality and the microbiological influence. It is equipped with different sampling systems to enable four variable operating modes. Both airborne microbes as well as chemical or particle airborne pollutants are collected. For that reason different air separation methods can be selected, such as impinger, filtration, adsorption, and absorption principles.